Journal: Journal of Nematology
Article Title: Heterorhabditis caligo n. sp. (Rhabditida: Heterorhabditidae): A New Entomopathogenic Nematode from Pichilemu Sand Dunes, Chile
doi: 10.2478/jofnem-2025-0045
Figure Lengend Snippet: Maximum aphylogenetic tree reconstructed from the concatenated sequences of the ITS region of the rRNA gene and the cytochrome c oxidase subunit I ( cox-1 ) gene of different Heterorhabditis species. A total of 1,482 nucleotide positions were analyzed. The ITS sequences of H. marelatus and H. mexicana were obtained from the NCBI using the accession numbers AY321479 and EF043444 , respectively. The ITS sequences of all the other isolates were extracted from whole ribosomal RNA operons. These sequences were then trimmed to obtain sequences that cover the region flanked by the commonly used primers TW81 and AW28. The cox-1 sequences of H. marelatus and H. mexicana were obtained from the NCBI using the accession numbers EF043419 and EF043422 , respectively. The sequences of all the other isolates were extracted from whole mitochondrial genomes. These sequences were then trimmed to obtain sequences that cover the region flanked by the commonly used primers HCF and HCR. Accession numbers of the nucleotide sequences used for the analyses are shown in in the . Numbers at nodes represent bootstrap values based on 500 replications. Bars represent average nucleotide substitutions per sequence position. ITS, internal transcribed spacer; NCBI, National Center for Biotechnology Information.
Article Snippet: The final assemblies were polished using Pilon 1.24 and the National Center for Biotechnology Information (NCBI) Foreign Contamination Screen (FCS) genome cross-species aligner (GX) tool (NCBI FCS GX v0.5.0) was used to remove scaffolds belonging to organisms other than Nematoda ( ; ).
Techniques: Sequencing
